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Medical Preparation Ensured informed consent is signed by both the parents after thorough explanation by the medical team Preoperative assessments including, Full blood work, Infectious disease screening, Cross matching Central line inserted under local anesthesia NPO status as per guidelines Administration of GCSF (Granulocyte – colony Stimulating Factor for 5days) and monitored closely for side effects that is bone pain, Low grade fever and irritability and provided analgesics accordingly Intra procedure Nursing care Assisted for IV sedation and provided emotional support to the child and parents Prepared and kept all emergency equipment Fluid replacement as per pediatric intensivist’s advice Maintained aseptic technique throughout the procedure Monitored vital signs & Hemodynamic status closely throughout the procedure Monitored closely for signs of hypocalcemia that is tingling, muscle cramps, irritability and administered calcium gluconate as prescribed appropriately to prevent such symptoms Kept the child warm and calm throughout the procedure Post procedure Nursing care Monitored vitals, Bleeding at harvest site, Pain and signs of infection closely and administered analgesics as prescribed by the pediatric intensivist

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