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#8823) at 4 °C for 2 h, and the beads were washed with lysis buffer five times, and Sirt7 buffer (50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM DTT, and 20% glycerol) three times, sequentially

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Acta 1820 (10), 1588–1596