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Protein (50 μg) was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis on a Precast PAGE Gel (4–12%, APPLYGEN, Beijing, China) and electroblotted onto nitrocellulose membranes

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Anyone with a current or recent cancer diagnosis should raise GHK-Cu specifically with their oncologist before use, given this exact gap between lab-level anti-cancer signal and the absence of long-term human safety trials